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Abstract
Abstract
Transplantation of Human Corneal Endothelial Cells as Potential Therapy of Corneal Endothelial Dystrophies
Aboalchamat B.1, Böhnke M.2, Engelmann K.3, Bednarz J.1
1Ophthalmology Department, Univerisity of Hamburg; 2Ophthalmic and Laser Center, Hamburg; 3Ophthalmology Department, University of Dresden
Purpose: Our previous studies showed that transplantation of human corneal endothelial cells onto organ cultured corneas led to the establishment of a morphological and functional intact endothelium. In this study we developed a new technique to transplant human corneal endothelial cells onto human corneas in whole globes.
Method: We used porcine eyes as well as human eyes that were not suitable for donor cornea preparation. Following paracentesis, the native corneal endothelium was removed by scratching with polishing cannulas and simultaneous rinsing with isotonic saline solution. Subsequently air was inflated into the anterior chamber. Suspensions of 250 000 cells of a SV40 transfected cell line in 150 ml culture medium were injected into the air inflated anterior chamber of donor globes with the cornea downside. The globes were kept in this position for different incubation periods. Subsequently the corneas were prepared for organ culture. The transplanted cells were identified by immunochemical proof of SV40 T-antigen expression and scanning electronmicroscopy.
Results: The use of polishing cannulas led to a complete removal of the native corneal endothelium